5U7Z
Human acid ceramidase (ASAH1, aCDase) self-activated
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-30 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 153.667, 68.312, 97.805 |
| Unit cell angles | 90.00, 120.72, 90.00 |
Refinement procedure
| Resolution | 48.389 - 2.500 |
| R-factor | 0.2354 |
| Rwork | 0.234 |
| R-free | 0.27090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.675 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.389 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 26671 |
| <I/σ(I)> | 10.8 |
| Completeness [%] | 99.0 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 1000 sodium phosphate-citrate lithium sulfate |
