5U7Z
Human acid ceramidase (ASAH1, aCDase) self-activated
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-30 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97949 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 153.667, 68.312, 97.805 |
Unit cell angles | 90.00, 120.72, 90.00 |
Refinement procedure
Resolution | 48.389 - 2.500 |
R-factor | 0.2354 |
Rwork | 0.234 |
R-free | 0.27090 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.675 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.389 |
High resolution limit [Å] | 2.500 |
Number of reflections | 26671 |
<I/σ(I)> | 10.8 |
Completeness [%] | 99.0 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 1000 sodium phosphate-citrate lithium sulfate |