5U78
Crystal structure of ORP8 PH domain in P1211 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.170, 66.350, 79.549 |
| Unit cell angles | 90.00, 94.67, 90.00 |
Refinement procedure
| Resolution | 40.927 - 1.978 |
| R-factor | 0.2244 |
| Rwork | 0.223 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5u77 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.245 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.930 |
| High resolution limit [Å] | 1.980 |
| Rmerge | 0.100 |
| Number of reflections | 37677 |
| <I/σ(I)> | 9.7 |
| Completeness [%] | 99.2 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M Bis-tris propane, pH 6.5, 0.2 M Potassium thiocyanate, 20% PEG3350 |
