5U66
Modified single helix from the B-domain of protein A bound to IgG1 Fc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 1997-05-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 49.165, 126.812, 91.927 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.020 - 1.700 |
R-factor | 0.164 |
Rwork | 0.157 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5u52 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.964 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.229 | |
Number of reflections | 29486 | |
<I/σ(I)> | 29.8 | 4.7 |
Completeness [%] | 96.4 | 93.2 |
Redundancy | 5.7 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 20% PEG 4000, 0.1M HEPES pH 7.2, 5mg/mL protein concentration. |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 20% PEG 4000, 0.1M HEPES pH 7.2, 5mg/mL protein concentration. |