5U66

Modified single helix from the B-domain of protein A bound to IgG1 Fc

Summary for 5U66

• Entry DOI: 10.2210/pdb5u66/pdb
• Related PRD ID: PRD_002261
• Descriptor: STAPLED PEPTIDE FROM DOMAIN B OF PROTEIN A, IgG1 fc derived from CD4-fc fusion, beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• Functional Keywords: igg1 fc, staple peptide, protein a, b-domain, immune system-inhibitor complex, immune system/inhibitor
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 2
• Total formula weight: 26896.14

Authors

Ultsch, M.H.,Eigenbrot, C. (deposition date: 2016-12-07, release date: 2017-05-24, Last modification date: 2023-11-15)

Primary citation

Ultsch, M.,Braisted, A.,Maun, H.R.,Eigenbrot, C.
3-2-1: Structural insights from stepwise shrinkage of a three-helix Fc-binding domain to a single helix.
Protein Eng. Des. Sel., 30:619-625, 2017

PubMed Abstract: The well-studied B-domain from Staphylococcal protein A is a 59 amino acid three-helix bundle that binds the Fc portion of IgG with a dissociation constant of ~35 nM. The B-domain variant bearing a Gly to Ala mutation (=Z-domain) has been the subject of efforts to minimize a domain's size while retaining its function. We report X-ray crystallographic characterization of three steps in such a process using complexes with Fc: the full three-helix Z-domain, a 34 amino acid two-helix version called Z34C and a 13 amino acid single helix stabilized with an exo-helix tether, called LH1.

PubMed: 28475752
DOI: 10.1093/protein/gzx029

Experimental method

X-RAY DIFFRACTION (1.7 Å)