5U18
Crystal structure of a methyltransferase involved in the biosynthesis of gentamicin in complex with the Geneticin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X3A |
Synchrotron site | NSLS |
Beamline | X3A |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2013-11-13 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.113, 68.892, 69.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.854 - 2.195 |
R-factor | 0.1731 |
Rwork | 0.171 |
R-free | 0.22790 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.854 |
High resolution limit [Å] | 2.195 |
Number of reflections | 16371 |
<I/σ(I)> | 13.64 |
Completeness [%] | 99.2 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | Magnesium chloride, tris pH 8, peg 3350 and peg 400 |