5TZF

Structure of the BldD CTD(D116A)-(c-di-GMP)2 intermediate, form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-21
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	87.133, 93.061, 94.094
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.166 - 2.400
R-factor	0.2315
Rwork	0.229
R-free	0.27190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tzd
RMSD bond length	0.011
RMSD bond angle	1.096
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.200	2.530
High resolution limit [Å]	2.400	2.400
Rmerge		0.320
Number of reflections	30442
<I/σ(I)>	12.4	3.6
Completeness [%]	99.6	68.3
Redundancy	3	3
CC(1/2)	0.998	0.908

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.6 M sodium/potassium phosphate, 100 mM Tris pH 7.5