5TVM
Crystal structure of Trypanosoma brucei AdoMetDC/prozyme heterodimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97932 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.297, 96.708, 99.584 |
Unit cell angles | 90.00, 102.64, 90.00 |
Refinement procedure
Resolution | 37.904 - 2.408 |
R-factor | 0.2307 |
Rwork | 0.229 |
R-free | 0.27350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5tvo |
RMSD bond length | 0.002 |
RMSD bond angle | 0.464 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 57780 | |
<I/σ(I)> | 15.1 | 1.3 |
Completeness [%] | 99.5 | 97.4 |
Redundancy | 6.9 | 5.6 |
CC(1/2) | 0.600 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 293 | 18 % PEG 6000, 0.1 M Bis-tris propane, pH 7.9 |