5TUF
Crystal structure of tetracycline destructase Tet(50) in complex with anhydrotetracycline
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2015-10-21 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.000031 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.990, 107.370, 152.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.791 - 2.250 |
R-factor | 0.2084 |
Rwork | 0.207 |
R-free | 0.25450 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.742 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1616) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 40157 | |
<I/σ(I)> | 13.57 | |
Completeness [%] | 98.6 | |
Redundancy | 4.93 | |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M MES, pH 6.0-6.5, 1.6-2.0 M ammonium sulfate, 2-10% 1,4-dioxane |