Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE M18X |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-12-01 |
| Detector | MACSCIENCE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.170, 94.560, 95.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 800.000 * - 2.500 |
| R-factor | 0.208 * |
| Rwork | 0.207 |
| R-free | 0.29900 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.992 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.01) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.072 * |
| Number of reflections | 41282 |
| Completeness [%] | 92.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 24 * | 20% POLYETHYLENEGLYCOL 4000, 0.1 M SODIUM CITRATE PH 5.6, pH 6.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 25 (%) | |
| 4 | 1 | reservoir | sodium citrate | 0.1 (M) |
