5TJH
hUGDH A136M Substitution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2015-11-14 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 92.350, 104.590, 107.710 |
| Unit cell angles | 64.82, 68.35, 73.72 |
Refinement procedure
| Resolution | 37.173 - 2.050 |
| R-factor | 0.1725 |
| Rwork | 0.171 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rjt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.893 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.180 |
| High resolution limit [Å] | 2.050 |
| Number of reflections | 206192 |
| <I/σ(I)> | 7.8 |
| Completeness [%] | 91.3 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 12% PEG 3350,0.2M NaCl, 0.1M Tris buffer pH 7.6 |
