5TJ2
Gasdermin-B C-terminal domain containing the polymorphism residues Gly299:Ser306 fused to maltose binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2015-11-14 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 144.039, 152.750, 255.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.500 - 2.800 |
R-factor | 0.2153 |
Rwork | 0.213 |
R-free | 0.25240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5tib |
RMSD bond length | 0.011 |
RMSD bond angle | 1.474 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 42.500 |
High resolution limit [Å] | 2.800 |
Rmerge | 0.155 |
Number of reflections | 65695 |
<I/σ(I)> | 11.3 |
Completeness [%] | 99.6 |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 295 | 2.1 M sodium malonate, 10 mM EDTA |