5TIO
Crystal Structure of Human Glycine Receptor alpha-3 Bound to AM-3607
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-13 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 119.120, 119.120, 429.968 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.250 |
R-factor | 0.234 |
Rwork | 0.233 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rif |
RMSD bond length | 0.006 |
RMSD bond angle | 1.048 |
Data scaling software | HKL-2000 (2.3.8) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.370 |
High resolution limit [Å] | 3.250 | 3.250 |
Rmerge | 0.131 | 1.061 |
Number of reflections | 47335 | |
<I/σ(I)> | 16.2 | 2.3 |
Completeness [%] | 99.8 | 98.6 |
Redundancy | 13.8 | 10.4 |
CC(1/2) | 0.999 | 0.740 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 200 mM Calcium Chloride, 22.5-27.5% PEG-350MME, 100 mM MES |