5TFG
Nucleotide-binding domain 1 of the human cystic fibrosis transmembrane conductance regulator (CFTR) with 5-methyl-UTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-28 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.18076 |
Spacegroup name | P 43 |
Unit cell lengths | 40.147, 40.147, 141.787 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.147 - 1.910 |
R-factor | 0.1591 |
Rwork | 0.156 |
R-free | 0.18360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pze |
RMSD bond length | 0.009 |
RMSD bond angle | 1.112 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.910 |
Number of reflections | 16965 |
<I/σ(I)> | 60.5 |
Completeness [%] | 97.7 |
Redundancy | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 279 | Protein buffer: 150 mM NaCl, 30% (v/v) glycerol, 1 mM TCEP, and 20 mM Na-HEPES, pH 7.5; Precipitant buffer: 40% (v/v) PEG 400, 100 mM NH4Cl, and 100 mM MES, pH 6 |