5TEF
Crystal structure of Gemin5 WD40 repeats in complex with m7GpppG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97944 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.658, 124.862, 60.938 |
| Unit cell angles | 90.00, 117.52, 90.00 |
Refinement procedure
| Resolution | 48.020 - 1.950 |
| R-factor | 0.1807 |
| Rwork | 0.179 |
| R-free | 0.21740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | "apo" GEMIN5 WD40 repeats |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.524 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.020 | 48.020 | 2.000 |
| High resolution limit [Å] | 1.950 | 8.940 | 1.950 |
| Rmerge | 0.079 | 0.042 | 0.675 |
| Rmeas | 0.092 | 0.049 | 0.781 |
| Rpim | 0.046 | 0.026 | 0.393 |
| Total number of observations | 216124 | 2154 | 15195 |
| Number of reflections | 55703 | ||
| <I/σ(I)> | 11 | 31.7 | 1.8 |
| Completeness [%] | 98.6 | 98.7 | 97.6 |
| Redundancy | 3.9 | 3.6 | 3.9 |
| CC(1/2) | 0.997 | 0.995 | 0.847 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 293 | 15% PEG4000, 0.2 M ammonium acetate, 0.1 sodium citrate, 0.0005-0.001 M m7GpppG |
