5TDF
TEV Cleaved Human ATP Citrate Lyase Bound to 4S hydroxycitrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.050, 83.870, 195.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.940 - 1.800 |
R-factor | 0.152 |
Rwork | 0.150 |
R-free | 0.19100 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.994 |
Data scaling software | Aimless |
Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 41.940 |
High resolution limit [Å] | 1.800 |
Number of reflections | 84385 |
<I/σ(I)> | 8.4 |
Completeness [%] | 99.6 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 294 | 15% P3350, 100 mM TrisHCl pH 8.4, 150 mM ammonium chloride |