5TCX
Crystal structure of human tetraspanin CD81
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2015-10-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.430, 73.510, 70.350 |
| Unit cell angles | 90.00, 125.11, 90.00 |
Refinement procedure
| Resolution | 36.755 - 2.955 |
| R-factor | 0.2695 |
| Rwork | 0.267 |
| R-free | 0.29350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g8q |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.589 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.755 | 3.030 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.180 | |
| Number of reflections | 5006 | |
| <I/σ(I)> | 3.5 | 0.3 |
| Completeness [%] | 98.6 | 95.5 |
| Redundancy | 4.9 | 3.2 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8 | 291 | 25-35% PEG300, Ammonium citrate dibasic, Tris |
