5TCG
Crystal structure of tryptophan synthase from M. tuberculosis - aminoacrylate-bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97926 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 135.183, 159.433, 165.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.400 |
R-factor | 0.1724 |
Rwork | 0.172 |
R-free | 0.20614 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.440 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.178 | 0.820 |
Number of reflections | 139260 | |
<I/σ(I)> | 11.78 | 1.81 |
Completeness [%] | 99.6 | 98.3 |
Redundancy | 8.9 | 6 |
CC(1/2) | 0.632 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 8% tacsimate pH 8.0, 20% PEG3350, 3% trimethylamine N-oxide dihydrate, soaked in 80 mM CsCl, 30 mM L-Ser and 17% ethylene glycol |