Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

5T7O

Crystal structure of Trypanosoma cruzi Dihydrofolate Reductase-Thymidylate Synthase in complex with (6S)-5,6,7,8-TETRAHYDROFOLATE

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsDIAMOND BEAMLINE I24
Synchrotron siteDiamond
BeamlineI24
Temperature [K]100
Detector technologyPIXEL
Collection date2015-12-07
DetectorDECTRIS PILATUS3 6M
Wavelength(s)0.96861
Spacegroup nameI 2 2 2
Unit cell lengths154.547, 173.540, 174.159
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution86.770 - 1.800
R-factor0.17906
Rwork0.177
R-free0.21991
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3inv
RMSD bond length0.026
RMSD bond angle2.392
Data reduction softwareiMOSFLM
Data scaling softwareSCALA
Phasing softwareMOLREP
Refinement softwareREFMAC (5.8.0135)
Data quality characteristics
 Overall
Low resolution limit [Å]86.770
High resolution limit [Å]1.800
Number of reflections213627
<I/σ(I)>9.2
Completeness [%]99.5
Redundancy6.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1MICROBATCH298TcDHFR-TS 12.5 mg ml-1 in 25 mMTris pH 7.2 and 80 mMNaCl, precipitant solution 12% (w/v) PEG 3350, 100 mM sodium malonate pH 5

227561

PDB entries from 2024-11-20

PDB statisticsPDBj update infoContact PDBjnumon