5T6B
X-ray structure of the KijD1 C3-methyltransfeerase, converted to monomeric form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-12 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.707, 82.455, 82.825 |
| Unit cell angles | 96.05, 110.70, 112.09 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.17855 |
| Rwork | 0.176 |
| R-free | 0.23424 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5t64 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.773 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.305 | |
| Number of reflections | 90291 | |
| <I/σ(I)> | 7.3 | 2.1 |
| Completeness [%] | 96.6 | 91 |
| Redundancy | 3.1 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 18-22% PEG-3350, 2%MPD, 100 mM MES, 10 mM TDP, 5 mM SAH |
