5T5C

A Novel domain in human EXOG converts apoptotic endonuclease to DNA-repair enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-09
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	73.499, 80.267, 139.177
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.231 - 1.851
R-factor	0.1886
Rwork	0.186
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ism
RMSD bond length	0.007
RMSD bond angle	0.787
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.880
High resolution limit [Å]	1.850	5.020	1.850
Rmerge	0.078	0.032
Rmeas	0.082	0.035
Rpim	0.032	0.014	0.433
Total number of observations	505427
Number of reflections	70495
<I/σ(I)>	8.2
Completeness [%]	99.9	98.8	99.1
Redundancy	7.2	6.7	6.3
CC(1/2)		0.999	0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	22% PEG 2K MME, 10mM MgCl2, 0.1M Na Acetate pH 4.6, 0.2M (NH4)2SO4