5T5C
A Novel domain in human EXOG converts apoptotic endonuclease to DNA-repair enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-09 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.499, 80.267, 139.177 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.231 - 1.851 |
| R-factor | 0.1886 |
| Rwork | 0.186 |
| R-free | 0.21570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ism |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.787 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 5.020 | 1.850 |
| Rmerge | 0.078 | 0.032 | |
| Rmeas | 0.082 | 0.035 | |
| Rpim | 0.032 | 0.014 | 0.433 |
| Total number of observations | 505427 | ||
| Number of reflections | 70495 | ||
| <I/σ(I)> | 8.2 | ||
| Completeness [%] | 99.9 | 98.8 | 99.1 |
| Redundancy | 7.2 | 6.7 | 6.3 |
| CC(1/2) | 0.999 | 0.852 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 22% PEG 2K MME, 10mM MgCl2, 0.1M Na Acetate pH 4.6, 0.2M (NH4)2SO4 |
