5T4I
A Novel domain in human EXOG converts apoptotic endonuclease to DNA-repair enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-29 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 1.89 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.508, 79.723, 138.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.031 - 2.389 |
R-factor | 0.1928 |
Rwork | 0.191 |
R-free | 0.22870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5t40 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.526 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
High resolution limit [Å] | 2.389 | 6.510 | 2.389 |
Rmerge | 0.114 | 0.057 | 0.638 |
Rmeas | 0.126 | ||
Rpim | 0.052 | ||
Total number of observations | 352305 | ||
Number of reflections | 32857 | ||
<I/σ(I)> | 8.2 | ||
Completeness [%] | 99.5 | 99.6 | 97.4 |
Redundancy | 5.7 | 6.2 | 4.4 |
CC(1/2) | 0.997 | 0.792 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | 22% PEG 2000 MM 0.2M (NH4)2SO4, 0.1M Na Acetate pH 4.6 100mM MnCl2 |