5T0U
CTCF ZnF2-7 and DNA complex structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-02 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.27046 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 101.630, 40.960, 106.670 |
| Unit cell angles | 90.00, 92.21, 90.00 |
Refinement procedure
| Resolution | 53.295 - 3.199 |
| R-factor | 0.2314 |
| Rwork | 0.230 |
| R-free | 0.26630 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.520 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.11_2558: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.400 | 3.280 |
| High resolution limit [Å] | 3.190 | 3.190 |
| Rmerge | 0.163 | 0.350 |
| Number of reflections | 27870 | |
| <I/σ(I)> | 8.2 | 2.34 |
| Completeness [%] | 99.3 | 94.3 |
| Redundancy | 2.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 15% PEG 4000, 0.2 M ammonium acetate, 0.2 M Na-Citrate pH 5.6 |
