5T0A

Crystal Structure of Heparan Sulfate 6-O-Sulfotransferase with bound PAP and heptasaccharide substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-08
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	75.775, 128.291, 178.929
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.842 - 1.950
R-factor	0.1809
Rwork	0.180
R-free	0.21310
RMSD bond length	0.008
RMSD bond angle	1.157
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.950
Number of reflections	126425
<I/σ(I)>	8.2
Completeness [%]	99.2
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	25mM Tris pH 7.5 200mM disodium tartrate 18-19% PEG3350 1mM PAP 10mM oligosaccharide 5mM maltose