5T01
Human c-Jun DNA binding domain homodimer in complex with methylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-27 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.871, 42.490, 45.167 |
| Unit cell angles | 90.00, 98.01, 90.00 |
Refinement procedure
| Resolution | 27.750 - 1.890 |
| R-factor | 0.1949 |
| Rwork | 0.193 |
| R-free | 0.22710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fos |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.374 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.960 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.083 | 0.800 |
| Number of reflections | 23735 | |
| <I/σ(I)> | 20.3 | 2.63 |
| Completeness [%] | 98.5 | 90.1 |
| Redundancy | 6.9 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 0.05 M Citric acid 0.05 M Bis-Tris propane 16% PEG3350 |
