5SBC

Tubulin-maytansinoid-5a-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-23
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00003
Spacegroup name	P 21 21 21
Unit cell lengths	104.911, 157.908, 181.461
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.020 - 2.320
R-factor	0.1878
Rwork	0.186
R-free	0.22790
Structure solution method	DIFFERENCE FOURIER
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.19.1_4122)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.020	48.020	2.380
High resolution limit [Å]	2.320	10.380	2.320
Rmerge	0.183	0.029	3.207
Rmeas	0.190	0.031	3.326
Total number of observations	1795135
Number of reflections	130943	1612	9577
<I/σ(I)>	12.81	69.39	0.85
Completeness [%]	100.0	98.7	100
Redundancy	13.709	12.661	14.263
CC(1/2)	0.999	1.000	0.357

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	3% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine