5SB7

Tubulin-todalam-18-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-26
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	105.148, 158.288, 179.988
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.080 - 2.100
R-factor	0.2034
Rwork	0.202
R-free	0.23500
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.19.1_4122)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.080	48.080	2.150
High resolution limit [Å]	2.100	9.390	2.100
Rmerge	0.074	0.020	1.221
Rmeas	0.077	0.021	1.271
Total number of observations	2363099
Number of reflections	174917	2147	12807
<I/σ(I)>	23.92	84.32	2.15
Completeness [%]	99.9	99	99.9
Redundancy	13.51	12.596	12.99
CC(1/2)	1.000	1.000	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine