5SB4

Tubulin-todalam-8-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-23
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00003
Spacegroup name	P 21 21 21
Unit cell lengths	104.575, 157.329, 180.728
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.620 - 2.500
R-factor	0.1999
Rwork	0.198
R-free	0.23020
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.19.1_4122)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.620	49.620	2.570
High resolution limit [Å]	2.500	11.190	2.500
Rmerge	0.277	0.043	3.265
Rmeas	0.287	0.045	3.395
Total number of observations	1401930
Number of reflections	103407	1288	7548
<I/σ(I)>	10.14	50.69	0.76
Completeness [%]	99.9	98.5	99.4
Redundancy	13.557	12.849	13.274
CC(1/2)	0.996	0.999	0.270

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine