5OYH
crystal structure of the catalytic core of a rhodopsin-guanylyl cyclase with converted specificity in complex with ATPalphaS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | I 41 |
Unit cell lengths | 193.283, 193.283, 225.501 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.897 - 2.249 |
R-factor | 0.1829 |
Rwork | 0.182 |
R-free | 0.22350 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 0.938 |
Data reduction software | XDS |
Phasing software | PHENIX (1.10.1_2155) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.918 | 48.918 | 2.370 |
High resolution limit [Å] | 2.240 | 6.660 | 2.240 |
Rmerge | 0.117 | 0.026 | 1.003 |
Rmeas | 0.125 | 0.028 | 1.074 |
Total number of observations | 1530638 | ||
Number of reflections | 197393 | 7511 | 31749 |
<I/σ(I)> | 15.17 | 52.3 | 2.04 |
Completeness [%] | 99.7 | 98.5 | 99.6 |
Redundancy | 7.754 | 7.458 | 7.857 |
CC(1/2) | 0.999 | 1.000 | 0.720 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2M Potassium thiocyanate 0.1M Sodium cacodylate pH 6.5 25% w/v PEG 2000 mme |