5OSC
GLIC-GABAAR alpha1 chimera crystallized in complex with pregnenolone sulfate at pH 4.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.310, 133.180, 161.860 |
| Unit cell angles | 90.00, 102.71, 90.00 |
Refinement procedure
| Resolution | 29.920 - 3.100 |
| R-factor | 0.213 |
| Rwork | 0.211 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5osa |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.530 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.520 | 3.050 |
| High resolution limit [Å] | 2.970 | 2.970 |
| Rmerge | 0.098 | |
| Number of reflections | 77782 | |
| <I/σ(I)> | 1 | |
| Completeness [%] | 99.4 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 12-16% PEG 4000, 100 mM NaCl, 100 mM Li2S04, 100 mM sodium acetate pH 4-5 |
