5OSB

GLIC-GABAAR alpha1 chimera crystallized in complex with THDOC at pH4.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.976
Spacegroup name	C 1 2 1
Unit cell lengths	185.218, 133.507, 162.263
Unit cell angles	90.00, 103.41, 90.00

Refinement procedure

Resolution	29.980 - 3.800
R-factor	0.244
Rwork	0.241
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5osa
RMSD bond length	0.001
RMSD bond angle	0.432
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.030	3.970
High resolution limit [Å]	3.800	3.800
Rmerge	0.185
Number of reflections	37899
<I/σ(I)>	4.6
Completeness [%]	99.9
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	289	12-16% PEG4000, 100mM NaCl, 100 mM Li2SO4, 100mM sodium acetate pH4-4.5