5OO6
Complex of human nuclear cap-binding complex with ARS2 C-terminal peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-13 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 70.520, 112.990, 270.980 |
Unit cell angles | 90.00, 90.30, 90.02 |
Refinement procedure
Resolution | 270.980 - 2.800 |
R-factor | 0.23236 |
Rwork | 0.231 |
R-free | 0.26798 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h2t |
RMSD bond length | 0.008 |
RMSD bond angle | 1.194 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 280.000 | 2.910 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 200837 | 21706 |
<I/σ(I)> | 6.63 | 1.2 |
Completeness [%] | 97.5 | 96.3 |
Redundancy | 2.08 | 2 |
CC(1/2) | 0.995 | 0.585 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | CBC was mixed with an access of ARS2(827-871) in the presence of 1 mM m7GTP and subjected to gel filtration (120 mM NaCl, 5 mM beta-mercaptoethanol, 20 mM HEPES pH 7.8). The complex was concentrated to 8 mg/ml. Crystals were obtained in mother liquor containing 0.1 M sodium acetate pH 5, 8% (v/v) MPD and 0.1 M guanidine hydrochloride at 20 C. |