5ONH
Quaternary complex of wild type E. coli leucyl-tRNA synthetase with tRNA(leu), leucyl-adenylate analogue, and post-transfer editing analogue of norvaline in the aminoacylation conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.8726 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 159.110, 70.380, 229.830 |
Unit cell angles | 90.00, 104.47, 90.00 |
Refinement procedure
Resolution | 222.540 - 3.100 |
R-factor | 0.22427 |
Rwork | 0.223 |
R-free | 0.25725 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4aq7 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.906 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 44997 | 4316 |
<I/σ(I)> | 7.8 | 1.2 |
Completeness [%] | 99.4 | 96.2 |
Redundancy | 3.8 | 3.5 |
CC(1/2) | 0.990 | 0.400 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M BIS-TRIS PH 5.5, 23-25% W/V PEG 3350, AND 200 MM AMMONIUM ACETATE |