5ON3
Quaternary complex of mutant T252A of E. coli leucyl-tRNA synthetase with tRNA(leu), leucyl-adenylate analogue, and post-transfer editing analogue of leucine in the aminoacylation conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-18 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.000, 68.700, 227.200 |
| Unit cell angles | 90.00, 102.80, 90.00 |
Refinement procedure
| Resolution | 221.550 - 3.100 |
| R-factor | 0.24293 |
| Rwork | 0.241 |
| R-free | 0.27753 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aq7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.895 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.230 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 43333 | 4935 |
| <I/σ(I)> | 5.2 | 1.1 |
| Completeness [%] | 98.6 | 98.5 |
| Redundancy | 3.5 | 3.2 |
| CC(1/2) | 0.970 | 0.390 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M BIS-TRIS PH 5.5, 23-25% W/V PEG 3350, AND 200 MM AMMONIUM ACETATE |
