5ON3

Quaternary complex of mutant T252A of E. coli leucyl-tRNA synthetase with tRNA(leu), leucyl-adenylate analogue, and post-transfer editing analogue of leucine in the aminoacylation conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, D)	Leucine--tRNA ligase	polymer	880	99486.0	2	UniProt (P07813) Pfam (PF09334) Pfam (PF13603) Pfam (PF00133) Pfam (PF08264)	Escherichia coli K-12	Leucyl-tRNA synthetase,LeuRS
2	B, D (B, E)	tRNA(leu)	polymer	87	28052.7	2	GenBank (1208670917)	Escherichia coli
3	E (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	F, H (A, D)	5'-O-(L-leucylsulfamoyl)adenosine	non-polymer		459.5	2	Chemie (LSS)
5	G, I (A, D)	(2~{S})-~{N}-[(2~{R},3~{R},4~{S},5~{R})-2-(6-aminopurin-9-yl)-5-(hydroxymethyl)-4-oxidanyl-oxolan-3-yl]-2-azanyl-4-methyl-pentanamide	non-polymer		379.4	2	Chemie (9YN)

Sequence modifications

A, D: 1 - 860 (UniProt: P07813)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 252	Thr 252	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	255077.3
Non-Polymers*	Number of molecules	5
	Total formula weight	1743.2
All*	Total formula weight	256820.5

*Water molecules are not included.