5OMV
Ternary complex of 9N DNA polymerase in the replicative state with two metal ions in the active site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-07 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.999973488495 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.688, 94.964, 158.735 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.482 - 2.003 |
R-factor | 0.1916 |
Rwork | 0.189 |
R-free | 0.23320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4k8x |
RMSD bond length | 0.008 |
RMSD bond angle | 0.924 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12rc0_2787: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.482 | 2.120 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmeas | 0.142 | 3.603 |
Number of reflections | 130652 | 21057 |
<I/σ(I)> | 7.88 | 0.37 |
Completeness [%] | 99.7 | 99.6 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M sodium acetate trihydrate pH 4.5, 6% isopropanol, 36% PEG 550 MME |