4K8X

Binary complex of 9N DNA polymerase in the replicative state

Summary for 4K8X

• Entry DOI: 10.2210/pdb4k8x/pdb
• Related: 1QHT 4K8Z
• Descriptor: DNA polymerase, DNA (5'-D(P*AP*AP*AP*GP*GP*CP*GP*CP*AP*GP*TP*TP*CP*GP*CP*G)-3'), DNA (5'-D(*CP*GP*CP*GP*AP*AP*CP*TP*GP*CP*GP*(DOC))-3'), ... (8 entities in total)
• Functional Keywords: dna polymerase, binary complex, transferase-dna complex, transferase/dna
• Biological source: Thermococcus
• Total number of polymer chains: 3
• Total formula weight: 100033.04

Authors

Betz, K.,Diederichs, K.,Marx, A. (deposition date: 2013-04-19, release date: 2013-06-26, Last modification date: 2024-10-16)

Primary citation

Bergen, K.,Betz, K.,Welte, W.,Diederichs, K.,Marx, A.
Structures of KOD and 9N DNA Polymerases Complexed with Primer Template Duplex
Chembiochem, 14:1058-1062, 2013

PubMed Abstract: Replicate it: Structures of KOD and 9°N DNA polymerases, two enzymes that are widely used to replicate DNA with highly modified nucleotides, were solved at high resolution in complex with primer/template duplex. The data elucidate substrate interaction of the two enzymes and pave the way for further optimisation of the enzymes and substrates.

PubMed: 23733496
DOI: 10.1002/cbic.201300175

Experimental method

X-RAY DIFFRACTION (2.28 Å)