5OLA
Structure of mitochondrial transcription elongation complex in complex with elongation factor TEFM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 224.530, 155.550, 164.190 |
| Unit cell angles | 90.00, 113.58, 90.00 |
Refinement procedure
| Resolution | 49.153 - 3.904 |
| R-factor | 0.2445 |
| Rwork | 0.243 |
| R-free | 0.27560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4boc TEFM CTD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.624 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.160 | 4.000 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.258 | 2.187 |
| Number of reflections | 46976 | 3366 |
| <I/σ(I)> | 9.72 | 1.44 |
| Completeness [%] | 99.6 | 97.7 |
| Redundancy | 13.12 | 12.5 |
| CC(1/2) | 0.997 | 0.545 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | BIS-TRIS, Ammonium Sulfate, PEG3350 |
