5OLA

Structure of mitochondrial transcription elongation complex in complex with elongation factor TEFM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Transcription elongation factor, mitochondrial	polymer	234	27471.8	4	UniProt (Q96QE5)	Homo sapiens (Human)
2	E, I (E, F)	DNA-directed RNA polymerase, mitochondrial	polymer	1088	123326.5	2	UniProt (O00411) Pfam (PF14700) Pfam (PF00940)	Homo sapiens (Human)	MtRPOL
3	F, J (N, G)	DNA (5'-D(P*AP*TP*GP*GP*TP*GP*TP*AP*AP*CP*GP*CP*CP*AP*GP*AP*CP*GP*AP*AP*C)-3')	polymer	34	10491.8	2		synthetic construct
4	G, K (R, H)	RNA (5'-R(P*GP*CP*GP*GP*CP*GP*CP*GP*C)-3')	polymer	14	4493.7	2		synthetic construct
5	H, L (T, I)	DNA (30-MER)	polymer	34	10394.6	2		synthetic construct

Sequence modifications

A, B, C, D: 136 - 360 (UniProt: Q96QE5)

PDB	External Database	Details
Met 135	-	initiating methionine
Leu 361	-	expression tag
Glu 362	-	expression tag
His 363	-	expression tag
His 364	-	expression tag
His 365	-	expression tag
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag

E, F: 151 - 1230 (UniProt: O00411)

PDB	External Database	Details
Met 143	-	initiating methionine
Gly 144	-	expression tag
His 145	-	expression tag
His 146	-	expression tag
His 147	-	expression tag
His 148	-	expression tag
His 149	-	expression tag
His 150	-	expression tag
Ala 555	Glu 555	conflict

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	407300.5
All*	Total formula weight	407300.5

*Water molecules are not included.