4BOC

Structure of mitochondrial RNA polymerase elongation complex

Summary for 4BOC

• Entry DOI: 10.2210/pdb4boc/pdb
• Descriptor: DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL, 5'-D(*CP*AP*TP*GP*GP*GP*GP*TP*AP*AP*TP*TP*AP*TP *TP*TP*CP*GP*AP*CP*GP*CP*CP*AP*GP*AP*CP*G)-3', 5'-R(*AP*GP*UP*CP*UP*GP*CP*GP*GP*CP*GP*CP*GP*CP)-3', ... (5 entities in total)
• Functional Keywords: transcription, rna polymerase, mitochondria, transferase, dna-rna hybrid
• Biological source: HOMO SAPIENS (HUMAN); More
• Cellular location: Mitochondrion: O00411
• Total number of polymer chains: 4
• Total formula weight: 145059.29

Authors

Schwinghammer, K.,Cheung, A.,Morozov, Y.,Agaronyan, K.,Temiakov, D.,Cramer, P. (deposition date: 2013-05-18, release date: 2013-09-25, Last modification date: 2023-12-20)

Primary citation

Schwinghammer, K.,Cheung, A.C.M.,Morozov, Y.I.,Agaronyan, K.,Temiakov, D.,Cramer, P.
Structure of Human Mitochondrial RNA Polymerase Elongation Complex
Nat.Struct.Mol.Biol., 20:1298-, 2013

PubMed Abstract: Here we report the crystal structure of the human mitochondrial RNA polymerase (mtRNAP) transcription elongation complex, determined at 2.65-Å resolution. The structure reveals a 9-bp hybrid formed between the DNA template and the RNA transcript and one turn of DNA both upstream and downstream of the hybrid. Comparisons with the distantly related RNA polymerase (RNAP) from bacteriophage T7 indicates conserved mechanisms for substrate binding and nucleotide incorporation but also strong mechanistic differences. Whereas T7 RNAP refolds during the transition from initiation to elongation, mtRNAP adopts an intermediary conformation that is capable of elongation without refolding. The intercalating hairpin that melts DNA during T7 RNAP initiation separates RNA from DNA during mtRNAP elongation. Newly synthesized RNA exits toward the pentatricopeptide repeat (PPR) domain, a unique feature of mtRNAP with conserved RNA-recognition motifs.

PubMed: 24096365
DOI: 10.1038/NSMB.2683

Experimental method

X-RAY DIFFRACTION (2.65 Å)