4BOC
Structure of mitochondrial RNA polymerase elongation complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-09-25 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | I 2 3 |
Unit cell lengths | 225.190, 225.190, 225.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.810 - 2.650 |
R-factor | 0.1808 |
Rwork | 0.179 |
R-free | 0.20900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3spa |
RMSD bond length | 0.009 |
RMSD bond angle | 1.030 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.800 | 2.720 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.120 | |
Number of reflections | 54985 | |
<I/σ(I)> | 18.9 | 1.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 20.7 | 20.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 8% PEG 4000, 200 MM SODIUM ACETATE, 100 MM TRISODIUM CITRATE (PH=5.5), 10% GLYCEROL, 10 MM DTT, pH 7.5 |