5OKT
Crystal structure of human Casein Kinase I delta in complex with IWP-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-22 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 49.110, 82.730, 89.640 |
Unit cell angles | 89.73, 74.14, 86.57 |
Refinement procedure
Resolution | 47.155 - 2.130 |
R-factor | 0.1939 |
Rwork | 0.193 |
R-free | 0.22060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5mqv |
RMSD bond length | 0.004 |
RMSD bond angle | 0.715 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.155 |
High resolution limit [Å] | 2.130 |
Number of reflections | 74219 |
<I/σ(I)> | 14.1 |
Completeness [%] | 98.0 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M ammonium sulfate, 0.1 M sodium acetate pH 5.0 and 5 % (w/v) PEG 2000 MME |