5OHK

Crystal structure of USP30 in covalent complex with ubiquitin propargylamide (high resolution)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979490
Spacegroup name	P 21 2 21
Unit cell lengths	50.917, 94.408, 96.115
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.352 - 2.340
R-factor	0.2308
Rwork	0.229
R-free	0.26050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hd5
RMSD bond length	0.003
RMSD bond angle	0.525
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.352	2.424
High resolution limit [Å]	2.340	2.340
Rmerge	0.170	0.715
Rpim	0.092	0.380
Number of reflections	20131	1978
<I/σ(I)>	4.61	1.86
Completeness [%]	99.2	98.9
Redundancy	4.2	4
CC(1/2)	0.983	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.4	291	10% (w/v) PEG 20000, 0.1 M sodium citrate pH 5.4, 0.2 M lithium sulfate