5OHK
Crystal structure of USP30 in covalent complex with ubiquitin propargylamide (high resolution)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-03 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979490 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 50.917, 94.408, 96.115 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 67.352 - 2.340 |
R-factor | 0.2308 |
Rwork | 0.229 |
R-free | 0.26050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hd5 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.525 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.352 | 2.424 |
High resolution limit [Å] | 2.340 | 2.340 |
Rmerge | 0.170 | 0.715 |
Rpim | 0.092 | 0.380 |
Number of reflections | 20131 | 1978 |
<I/σ(I)> | 4.61 | 1.86 |
Completeness [%] | 99.2 | 98.9 |
Redundancy | 4.2 | 4 |
CC(1/2) | 0.983 | 0.848 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 291 | 10% (w/v) PEG 20000, 0.1 M sodium citrate pH 5.4, 0.2 M lithium sulfate |