5OC7
Crystal structure of the pleckstrin-homology domain of Bcr-Abl in complex with monobody Mb(Bcr-PH_4).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-19 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.999870 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.683, 62.606, 67.378 |
| Unit cell angles | 62.74, 84.77, 89.29 |
Refinement procedure
| Resolution | 33.680 - 1.652 |
| R-factor | 0.1754 |
| Rwork | 0.174 |
| R-free | 0.20430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dfk |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.076 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.680 | 1.711 |
| High resolution limit [Å] | 1.652 | 1.652 |
| Rmerge | 0.031 | 0.633 |
| Rmeas | 0.035 | 0.716 |
| Rpim | 0.016 | 0.328 |
| Number of reflections | 49951 | 4899 |
| <I/σ(I)> | 23.01 | |
| Completeness [%] | 97.1 | 96.1 |
| Redundancy | 4.899 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Potassium thiocyanate, 30% (w/v) PEG MME 2000 |
