5OC7

Crystal structure of the pleckstrin-homology domain of Bcr-Abl in complex with monobody Mb(Bcr-PH_4).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-19
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.999870
Spacegroup name	P 1
Unit cell lengths	29.683, 62.606, 67.378
Unit cell angles	62.74, 84.77, 89.29

Refinement procedure

Resolution	33.680 - 1.652
R-factor	0.1754
Rwork	0.174
R-free	0.20430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dfk
RMSD bond length	0.011
RMSD bond angle	1.076
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.680	1.711
High resolution limit [Å]	1.652	1.652
Rmerge	0.031	0.633
Rmeas	0.035	0.716
Rpim	0.016	0.328
Number of reflections	49951	4899
<I/σ(I)>	23.01
Completeness [%]	97.1	96.1
Redundancy	4.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Potassium thiocyanate, 30% (w/v) PEG MME 2000