5O9M
Crystal structure of human Histamine-Releasing Factor (HRF/TCTP)containing a disulphide-linked dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-23 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.92 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.527, 77.592, 99.325 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.855 - 1.400 |
R-factor | 0.1803 |
Rwork | 0.179 |
R-free | 0.19850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yz1 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.546 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.860 | 1.430 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.066 | 1.112 |
Rpim | 0.025 | 0.428 |
Number of reflections | 72800 | 3759 |
<I/σ(I)> | 12.7 | |
Completeness [%] | 99.7 | 99.6 |
Redundancy | 7.4 | 7.6 |
CC(1/2) | 0.999 | 0.703 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 0.1M MES pH6.0 and 20% (w/v) PEG MME 2000 |