5O90
Crystal structure of a P38alpha T185G mutant in complex with TAB1 peptide.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-01-31 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.920, 73.580, 58.970 |
Unit cell angles | 90.00, 91.09, 90.00 |
Refinement procedure
Resolution | 58.959 - 2.490 |
R-factor | 0.2502 |
Rwork | 0.249 |
R-free | 0.27480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4loo |
RMSD bond length | 0.010 |
RMSD bond angle | 1.179 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.960 | 2.320 |
High resolution limit [Å] | 2.260 | 2.260 |
Rmerge | 0.054 | 0.852 |
Number of reflections | 18410 | 1340 |
<I/σ(I)> | 22 | 1.1 |
Completeness [%] | 99.5 | 93.6 |
Redundancy | 9.4 | 3.7 |
CC(1/2) | 0.999 | 0.651 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 20% PEG3350 0.2M Na/K Tartrate 0.1M pH7.0 Bis-Tris Propane |