5O76
Structure of phosphoY371 c-CBL in complex with ZAP70-peptide and UbV.pCBL ubiquitin variant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2016-10-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97632 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 94.790, 101.281, 117.339 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.340 - 2.473 |
R-factor | 0.22 |
Rwork | 0.218 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4a4b |
RMSD bond length | 0.008 |
RMSD bond angle | 0.950 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 101.281 | 2.515 |
High resolution limit [Å] | 2.473 | 2.473 |
Rmerge | 0.112 | 1.155 |
Rpim | 0.066 | 0.667 |
Number of reflections | 38315 | 2007 |
<I/σ(I)> | 13.9 | 2.1 |
Completeness [%] | 93.3 | 99 |
Redundancy | 6.5 | 6.9 |
CC(1/2) | 0.998 | 0.796 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M monosaccharides, 0.1 M Buffer 1 pH 6.5, 50% Precipitant 2, Morpheus screen |