5O4S
Crystal structure of the human BRPF1 bromodomain in complex with BZ135
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-08 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.700, 62.690, 48.820 |
Unit cell angles | 90.00, 101.81, 90.00 |
Refinement procedure
Resolution | 38.000 - 1.750 |
R-factor | 0.181 |
Rwork | 0.178 |
R-free | 0.22070 |
Starting model (for MR) | 4lc2 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.722 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.000 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.151 | 0.730 |
Number of reflections | 29072 | 1578 |
<I/σ(I)> | 22.6 | 4.1 |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 13.2 | 12.9 |
CC(1/2) | 0.985 | 0.961 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 297 | 0.1 M Bis-tris propane, pH6.5, 0.15 M Sodium Nitrate, 20% PEG3350 |