5O4A
Human FGF in complex with a covalent inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92819 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 211.140, 57.270, 66.800 |
| Unit cell angles | 90.00, 107.57, 90.00 |
Refinement procedure
| Resolution | 63.680 - 2.010 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.22500 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.260 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.6) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.680 | 2.060 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.030 | 0.858 |
| Number of reflections | 50255 | 12626 |
| <I/σ(I)> | 15.1 | 1.4 |
| Completeness [%] | 98.9 | 98.9 |
| Redundancy | 3.3 | 3.4 |
| CC(1/2) | 0.999 | 0.683 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 18-20% (w/v) PEG8000, 200mM ammonium sulphate, 100mM PCTP pH 6.75 and 20% (v/v) ethylene glycol |
