5O44
Crystal structure of unbranched mixed tri-Ubiquitin chain containing K48 and K63 linkages.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-26 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0080 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 110.767, 110.767, 417.004 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 95.930 - 3.140 |
R-factor | 0.21866 |
Rwork | 0.217 |
R-free | 0.25405 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3b08 |
RMSD bond length | 0.017 |
RMSD bond angle | 2.376 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 104.250 | 3.330 |
High resolution limit [Å] | 3.140 | 3.140 |
Number of reflections | 27681 | |
<I/σ(I)> | 9.7 | 0.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.4 | 10 |
CC(1/2) | 0.675 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.3M MgSo4 and 100mM MES monohydrate pH 6.5 |