5O44
Crystal structure of unbranched mixed tri-Ubiquitin chain containing K48 and K63 linkages.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0080 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 110.767, 110.767, 417.004 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 95.930 - 3.140 |
| R-factor | 0.21866 |
| Rwork | 0.217 |
| R-free | 0.25405 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b08 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.376 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.250 | 3.330 |
| High resolution limit [Å] | 3.140 | 3.140 |
| Number of reflections | 27681 | |
| <I/σ(I)> | 9.7 | 0.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.4 | 10 |
| CC(1/2) | 0.675 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.3M MgSo4 and 100mM MES monohydrate pH 6.5 |
