5O44

Crystal structure of unbranched mixed tri-Ubiquitin chain containing K48 and K63 linkages.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003723	molecular_function	RNA binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0016567	biological_process	protein ubiquitination
A	0019941	biological_process	modification-dependent protein catabolic process
A	0031386	molecular_function	protein tag activity
A	0031625	molecular_function	ubiquitin protein ligase binding
A	0046872	molecular_function	metal ion binding
E	0003723	molecular_function	RNA binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0016567	biological_process	protein ubiquitination
E	0019941	biological_process	modification-dependent protein catabolic process
E	0031386	molecular_function	protein tag activity
E	0031625	molecular_function	ubiquitin protein ligase binding
E	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	binding site for residue SO4 A 101

Chain	Residue
A	ARG42
A	ARG72
A	ARG74

---

site_id	AC2
Number of Residues	5
Details	binding site for residue SO4 A 102

Chain	Residue
A	ARG72
E	ARG42
E	GLN49
E	ARG72
E	HOH206

---

site_id	AC3
Number of Residues	2
Details	binding site for residue MG A 103

Chain	Residue
A	THR66
A	GLU64

---

site_id	AC4
Number of Residues	3
Details	binding site for residue SO4 C 101

Chain	Residue
C	ARG42
C	GLN49
D	ARG42

---

site_id	AC5
Number of Residues	2
Details	binding site for residue SO4 C 102

Chain	Residue
C	ARG54
F	LYS11

---

site_id	AC6
Number of Residues	3
Details	binding site for residue MG D 101

Chain	Residue
D	THR55
D	SER57
D	ASP58

---

site_id	AC7
Number of Residues	4
Details	binding site for residue SO4 B 101

Chain	Residue
A	GLN62
B	ARG54
B	ASP58
D	GLY10

---

site_id	AC8
Number of Residues	6
Details	binding site for residue SO4 B 102

Chain	Residue
B	ARG42
B	GLN49
B	ARG72
F	ARG42
F	GLN49
F	ARG72

---

site_id	AC9
Number of Residues	7
Details	binding site for residue SO4 E 101

Chain	Residue
E	ILE44
E	ALA46
E	GLY47
E	HIS68
F	PHE45
F	SER65
F	THR66

---

site_id	AD1
Number of Residues	6
Details	binding site for residue SO4 E 102

Chain	Residue
A	ILE44
A	GLY47
A	HIS68
D	SER65
E	ARG72
E	ARG74

---

site_id	AD2
Number of Residues	5
Details	binding site for residue SO4 E 103

Chain	Residue
A	LEU73
A	ARG74
E	THR9
E	GLU34
E	HOH201

---

site_id	AD3
Number of Residues	4
Details	binding site for residue MG E 104

Chain	Residue
D	SER57
E	GLU24
E	ASP39
E	ASP52

---

site_id	AD4
Number of Residues	19
Details	binding site for Di-peptide LYS B 48 and GLY F 76

Chain	Residue
B	ILE44
B	PHE45
B	ALA46
B	GLY47
B	GLN49
B	LEU50
B	TYR59
C	ALA46
D	LEU71
F	ILE44
F	PHE45
F	ALA46
F	GLY47
F	GLN49
F	LEU50
F	LEU71
F	LEU73
F	ARG74
F	GLY75

---

Functional Information from PROSITE/UniProt

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGcqleD

Chain	Residue	Details
A	LYS27-ASP52
C	LYS27-ASP52
D	LYS27-ASP52

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	SITE: Interacts with activating enzyme

Chain	Residue	Details
D	ARG54
D	ARG72
F	ARG54
F	ARG72

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	SITE: Essential for function

Chain	Residue	Details
D	HIS68
F	HIS68

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25527291

Chain	Residue	Details
D	SER65
F	SER65

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: (Microbial infection) ADP-ribosylthreonine => ECO:0000269|PubMed:32330457

Chain	Residue	Details
D	THR66
F	THR66

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: ADP-ribosylglycine => ECO:0000269|PubMed:28525742

Chain	Residue	Details
D	GLY76
F	GLY76

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603

Chain	Residue	Details
D	LYS6
F	LYS6
B	LYS63

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Chain	Residue	Details
C	LYS11
D	GLY76
B	LYS11
F	GLY76

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144

Chain	Residue	Details
D	LYS11
D	ARG48
F	LYS11
F	ARG48

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860

Chain	Residue	Details
D	LYS27
F	LYS27

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127

Chain	Residue	Details
D	LYS29
F	LYS29

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577

Chain	Residue	Details
D	LYS33
F	LYS33

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106

Chain	Residue	Details
D	LYS63
F	LYS63

---